This is a pretty cut-and-dried one, so it'll be quick.
In the Batman: The Animated Series episode "Harley and Ivy" (1993), we learn that Harley Quin has two pet hyenas named Bud and Lou, after Bud Abbot and Lou Costello.
Not long after Harley Quinn got her own series, Bud and Lou appeared as well, in Harley Quinn #4 (January 2001).
And, although this is a bit surprising, they survived the New 52, appearing here on the cover of her first issue (September 2011), although they didn't actually appear within a New 52 comic until Suicide Squad #15 (December 2012).
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